Effect of Three Commercial Mouth Rinses on Epithelial Cells: an in Vitro Study

Aim: The aim of the present study was to determine the cytotoxic effect of three commercial mouth washes (chlorhexidine, persica and Irsha) upon Hela cells. Methods and Material: For determination cytotoxicity of Irsha, Chlorhexidine and Persica, uninfected cells were grown in the absence and presence of various dilutions (1:2 to 1:128) of these mouth wash at different times (1,2,5,15 and 60 minutes). Results: In this study, three mouth washes show cytotoxic effect on cultured cells, at commercially available concentration an even diluted and Irsha was the most toxic one. Cytotoxicity of three mouth washes reduced with decreasing concentration. Conclusion: Our results showed that all three solutions were toxic to Hela cells. On the basis of these results, we suggest that clinical application of these mouthwashes should be limited.